摘要： 随着环境基因组学及深度测序技术的发展,基于16S rRNA基因序列研究微生物种群结构取得了长足进展。然而,由于环境样本的复杂性,尤其缺少真实背景信息,定量研究环境微生物种群结构仍是当前的研究难点。测序算法仿真平台研究,不仅有助于定量、定性分析微生物种群组成及结构,而且有助于建立基准数据库来评价当前微生物数据分析算法。分别基于易错PCR误差模型和正态分布过程,模拟454测序仪乳液PCR过程及边合成边测序过程,提出454测序仪模拟测序算法 (Tsim) 。仿真结果表明:该模拟算法能较好地模拟454测序过程。
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